FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74960935
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA750950.010.82442794-95
ACACGTC1113600.09.95912194-95
ACGTCTG920350.09.67337794-95
CACACGT1322200.09.40672294-95
CACGTCT1071300.09.26418894-95
TCGGAAG3325950.08.75995494-95
ATCGGAA3501350.08.73709994-95
CGGAAGA3129550.08.691627594-95
GATCGGA3861500.08.54239894-95
GCACACG1600950.08.532989594-95
AGATCGG4308400.08.38854394-95
GTCTGAA917050.08.21099994-95
TCTGAAC838550.08.10435194-95
AGCACAC1916750.08.03113994-95
TAGATCG1176200.07.92159794-95
AGAGCAC2312400.07.9030394-95
CAGATCG1699650.07.65473594-95
GAGCACA2197300.07.549366594-95
AAGAGCA2728200.07.514100694-95
GAAGAGC3104200.07.39582694-95
Produced by FastQC (version 0.11.5)