FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80479398
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA592600.010.6392494-95
ACACGTC865500.09.56518894-95
ACGTCTG739700.09.22467394-95
GATCGGA2986850.09.02902794-95
ATCGGAA2696300.09.02412294-95
CACACGT1059400.08.91879994-95
CACGTCT864050.08.75084394-95
TCGGAAG2579600.08.74650194-95
CGGAAGA2449900.08.66732694-95
AGATCGG3347950.08.64647694-95
GCACACG1287900.08.188868594-95
TAGATCG1126350.08.17888794-95
CAGATCG1342550.07.81260594-95
AGAGCAC1913250.07.6690594-95
AGCACAC1600500.07.47166594-95
GTCTGAA801950.07.225863594-95
GAGCACA1872600.07.13691794-95
AAGATCG835250.07.027477794-95
AAGAGCA2295650.07.0141694-95
TCTGAAC737600.06.98117694-95
Produced by FastQC (version 0.11.5)