FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76433607
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA819800.010.95327894-95
ACACGTC1225150.010.48290294-95
ACGTCTG992650.010.32903594-95
CACGTCT1166050.09.92400394-95
CACACGT1456500.09.86353994-95
GCACACG1735150.09.08065494-95
ATCGGAA4008450.08.862164594-95
TCGGAAG3772450.08.75457794-95
CGGAAGA3520850.08.62566294-95
GATCGGA4414700.08.62094994-95
GTCTGAA998400.08.52503594-95
AGAGCAC2555200.08.41586694-95
AGATCGG4918250.08.39246194-95
TCTGAAC910900.08.36572194-95
AGCACAC2092250.08.29965594-95
TAGATCG1372950.08.25887394-95
GAGCACA2426700.08.07914994-95
AAGAGCA3045850.07.886327394-95
GAAGAGC3460200.07.658294-95
CAGATCG1889900.07.63930894-95
Produced by FastQC (version 0.11.5)