FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52967890
Sequences flagged as poor quality0
Sequence length32-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1710470.3229258329905156No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1074910.2029361562259701No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA640350.1208939982317589No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG617760.11662915022667505No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCT554040.10459922039560193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG108500.030.0260456
TCGACGA114750.028.41187
CGACGAG117200.027.4915038
GTTCGAC120550.026.8752525
CGGTATA167750.026.39458820
AGTTCGA138300.023.7352624
CAGTTCG144150.023.3651263
ACGAGGA177550.022.96251110
GACGAGG176450.022.8761359
GTATAGA215900.021.85245122
CGAGGAT173150.021.55786511
ATGACGG194350.021.3118516
TATAGAG230500.020.75144623
GAACGAT462200.020.57631745
ATAGAGT231350.020.43221524
ACCGCGT468350.019.8158516
GACCGCG469450.019.7261925
AGACCGC477400.019.6382164
CAGACCG484600.019.4300083
CCGCGTT482900.019.2198437
Produced by FastQC (version 0.11.5)