FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177014628
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG112550.011.19263445
TTACGCA123150.010.00192534
CGTATGC136800.09.61805446
TAGATCG235900.09.46280570
TCCGATC166750.09.4430396
GATCGGA596000.09.33711370
AGATCGG737900.09.16100570
ATCGGAA541000.08.91959370
TACGCAC147300.08.5997335
CTCGTAT147750.08.573444
TTCCGAT189000.08.4979615
TATGCCG157850.08.13593248
CTTACGC177700.07.14822433
CCGATCT251850.06.69684367
TCTCGTA220450.05.999997643
ACTTACG247350.05.48911232
AAGATCG472600.05.353673570
TCGGAAG780800.05.331937370
CGATCTA82150.05.23917158
CGCACAT265500.05.04796737
Produced by FastQC (version 0.11.5)