FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77113528
Sequences flagged as poor quality0
Sequence length25-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA737150.011.17913794-95
ACACGTC1085550.010.16086794-95
ACGTCTG907900.09.76332194-95
CACACGT1297050.09.25962794-95
CACGTCT1066350.09.12561894-95
ATCGGAA3350250.08.71896494-95
GTCTGAA877000.08.53352594-95
GCACACG1569600.08.531194-95
GATCGGA3697200.08.43171794-95
TCGGAAG3200350.08.3780694-95
TCTGAAC790100.08.35276694-95
CGGAAGA3025450.08.33431194-95
AGATCGG4121000.08.24852494-95
AGAGCAC2284650.08.12650794-95
AGCACAC1908300.08.11247594-95
TAGATCG1119300.07.94006594-95
GAGCACA2208850.07.68469594-95
AAGAGCA2661150.07.625084494-95
CAGATCG1537700.07.561585494-95
AAGATCG1131250.07.276908494-95
Produced by FastQC (version 0.11.5)