FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75589959
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA946500.09.40003694-95
GATCGGA1025050.09.28866394-95
TAGATCG346250.09.24646394-95
AGATCGG1179900.09.06674194-95
TCGGAAG978800.08.46974494-95
CGGAAGA957700.07.65314994-95
CAGATCG513050.07.32801294-95
ACACGTC350300.07.13591994-95
AAGATCG422400.07.034717694-95
CGTCTGA262800.06.786355594-95
CACACGT462150.06.5105794-95
GCACACG560200.06.38774494-95
ACGTCTG336100.06.247763694-95
CACGTCT392950.06.06126594-95
AGAGCAC948900.05.365658894-95
GAGATCG507900.05.25581494-95
Produced by FastQC (version 0.11.5)