FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57040784
Sequences flagged as poor quality0
Sequence length50-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT184100.010.619881
ATAGGGG116000.010.59085238
GGCATAG117650.010.40404135
TCTATAG270500.010.0058033
AGCTTAG130800.09.54858445
CTATAGT293050.09.3049594
ATAGTGT305550.09.2856256
CATAGGG131450.09.27772337
TTACTAG298800.09.23131414
TATAGTG308000.09.0509545
TACTAGA308400.08.95125215
TTCTATA321450.08.930812
GCATAGG139650.08.86157936
TAGTGTG320350.08.7231877
GCACCGA312950.08.621844
TTACGCG12850.08.58001535
CACCGAA314350.08.56192545
GGGAGGT239900.08.5342383
GTTACTA328400.08.50887313
GTCACTC209150.08.3049648
Produced by FastQC (version 0.11.5)