FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61618567
Sequences flagged as poor quality0
Sequence length31-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT640670.10397353122476867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG25900.017.809126
GAACGAT183800.017.7139645
AGACCGC179650.017.646684
GACCGCG181500.017.2192695
ACCGCGT182800.017.1952556
CAGACCG195300.016.5437893
CCGCGTT197300.015.9540377
GTCCGTT46450.015.5497941
AACGCTA162050.015.522428523
ACGCTAC164700.015.23169324
CGCGTTC210300.014.9999358
CGCTACC168850.014.83067825
CGTTACG34150.014.56023821
CCGGCTT212850.014.4193622
TCACGCT184400.014.3369778
AGAACGA233300.014.01322944
CCCGTAT40050.013.9894981
TTCACGC196850.013.8073867
ACGAACG33600.013.59391231
TCGACGA34800.013.5128517
Produced by FastQC (version 0.11.5)