FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60295982
Sequences flagged as poor quality0
Sequence length49-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT802580.13310671347885172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG45700.025.8328976
CGGTATA75650.021.49113820
TCGACGA56750.021.2783747
CGACGAG56000.020.7200938
GTTCGAC57150.020.4211965
GACCGCG216300.019.4304495
ACCGCGT217650.019.2789346
AGACCGC227650.018.7580364
CCGCGTT230100.018.2553547
CGCGTTC238200.017.7006638
CAGACCG246900.017.5875593
AACGCTA124850.016.62429423
ACGAGGA92750.016.55910510
GTTCCTA128400.016.28725815
ACGCTAC127800.016.15257824
TCAGACC295200.015.7003052
CGCTACC131600.015.66907925
AGTTCGA80400.015.0192524
CCTACTC140650.014.820744518
CAGTTCG86400.014.7943713
Produced by FastQC (version 0.11.5)