FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60295982
Sequences flagged as poor quality0
Sequence length50-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG151700.011.52484138
GGCATAG150600.010.98149635
GTTCTAT190950.010.7573031
TCTATAG313050.010.7227813
AGCTTAG162200.010.35011345
CTATAGT334650.010.0239724
CATAGGG173750.09.85511937
ATAGTGT350050.09.7762916
TATAGTG348950.09.7489655
GGGAGGT276150.09.7440263
TTACTAG344100.09.73559214
AACTCTA142850.09.71990545
TTCTATA364550.09.670842
TACTAGA351200.09.53236715
GTCACTC246650.09.4960858
GCATAGG179700.09.47842536
GGAGGTC273550.09.203364
GCACCGA349150.09.18460244
TAGTGTG367300.09.1823887
GTTGTCG34600.09.16843826
Produced by FastQC (version 0.11.5)