FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61757652
Sequences flagged as poor quality0
Sequence length31-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29000.022.2674086
TCGACGA36050.018.0997037
GAACGAT175300.017.82834445
ACCGCGT169400.017.6654226
AGACCGC173600.017.5358224
GACCGCG173700.017.1892765
CCGCGTT179050.016.687937
CGCGTTC180200.016.6064038
CGGTATA53600.015.78307420
CAGACCG198000.015.6250293
AACGCTA144850.015.03574923
GTTCCTA116250.014.88338115
ACGCTAC146650.014.86654124
CGACGAG42650.014.71851358
CGTTACG33050.014.56836621
GTTCGAC43850.014.5212285
CCCGTAT41550.014.3507641
CGCTACC152900.014.30299525
CCGGCTT193950.013.8056672
AGAACGA228200.013.79390144
Produced by FastQC (version 0.11.5)