FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104221714
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA64000.010.11350958
GATCGGA394350.010.07846570
AGATCGG487000.09.92990670
CGTATCA70950.09.32013661
GCCGTAT71750.09.21618459
ATCGGAA353900.09.21190670
TAGATCG166750.08.86190370
CCGTATC96600.06.91778860
TCGTGTA113000.06.006976614
TCGGAAG478400.05.987458770
CAGATCG274400.05.372524770
TCGCCGT128550.05.361683457
AAGATCG318850.05.150707270
CGGAAGA488500.05.039311470
Produced by FastQC (version 0.11.5)