FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77904390
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1907100.09.37449694-95
GATCGGA2102650.09.2588194-95
CGTCTGA429950.09.22477394-95
ACACGTC626050.09.19694394-95
AGATCGG2379700.08.96398694-95
TCGGAAG1854900.08.81842794-95
TAGATCG702850.08.79031494-95
ACGTCTG542700.08.70409794-95
CACACGT768400.08.4452794-95
CGGAAGA1774650.08.38295894-95
CACGTCT638400.08.13020794-95
GCACACG920200.07.881578494-95
AAGATCG723250.07.749864694-95
CAGATCG938500.07.64809894-95
AGAGCAC1452400.07.07584594-95
AGCACAC1247250.06.64163694-95
AAGAGCA1820700.06.47821394-95
GAAGAGC2047350.06.394130794-95
GAGATCG812950.06.37392694-95
GAGCACA1455100.06.36396694-95
Produced by FastQC (version 0.11.5)