FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80866730
Sequences flagged as poor quality0
Sequence length25-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA693600.010.33342294-95
ACACGTC1024200.09.66943294-95
ACGTCTG853250.09.47452594-95
CACGTCT1003400.08.97916494-95
CACACGT1229700.08.86735594-95
ATCGGAA3122400.08.75840494-95
GATCGGA3448750.08.75485994-95
AGATCGG3866650.08.6314894-95
CGGAAGA2824200.08.44705294-95
TCGGAAG2981150.08.40691694-95
TAGATCG1099850.08.36531294-95
GCACACG1486350.08.18075994-95
AGAGCAC2164250.07.898270694-95
CAGATCG1440950.07.848376394-95
GTCTGAA884250.07.824166394-95
AGCACAC1811450.07.67697494-95
TCTGAAC802950.07.568145894-95
AAGATCG1113700.07.56007294-95
GAGCACA2096550.07.39730594-95
AAGAGCA2571600.07.288254794-95
Produced by FastQC (version 0.11.5)