FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78114954
Sequences flagged as poor quality0
Sequence length50-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG46100.024.9411756
TCGACGA56950.020.4254467
CGGTATA81900.019.5600920
CGACGAG61800.018.2035588
ACCGCGT152900.017.5414586
GACCGCG153850.017.3893475
CCGCGTT164250.016.2462837
AGACCGC167500.016.0797214
GTTCGAC70900.015.9949625
CCCGTAT54350.015.6907041
CGCGTTC171900.015.5756368
CAGACCG182200.015.0664783
GTTCCTA139000.014.989140515
GAACGAT191950.014.70124845
AACGACG38050.014.42821716
TTCGGCG111500.014.20602344
TCGGCGC112800.014.12436645
CGTACGC111450.014.0314855
ACGAGGA113250.013.76814910
CCTACTC151000.013.69346218
Produced by FastQC (version 0.11.5)