FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106108593
Sequences flagged as poor quality0
Sequence length76
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1117500.09.71408470
GATCGGA1289650.09.60940370
AGATCGG1522200.09.37440170
TAGATCG451450.09.18395470
TCGGAAG1185500.07.90150670
CGTATCA62400.07.569347461
CGGAAGA1111350.07.357388570
GCCGTAT67600.07.142391759
AGAGCGT568150.06.95238470
AAGATCG683550.06.91593970
CAGATCG581950.06.913882370
CGCCGTA70450.06.903134358
AGCGTCG405050.06.80383570
GCGTCGT340050.06.703874670
AAGAGCG742150.06.61049770
CGTCGTG342900.05.677994770
GTCGTGT291750.05.629238670
GAGCGTC620150.05.601477670
TCGTGTA234550.05.37470670
CTAGATC299950.05.183483670
Produced by FastQC (version 0.11.5)