FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109249420
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC104750.010.8899866
TCGTATG72250.010.60674645
AGATCGG821250.010.4119670
CGTCGAA82050.09.97975536
GATCGGA680450.09.97588470
CCGTCGA77600.09.96573735
CGTATGC81150.09.7887646
ATCGGAA604950.09.75529970
TAGATCG267000.09.437036570
TTCCGAT127950.08.91540055
TATGCCG89350.08.65541248
CTCGTAT96200.08.03872844
CCGATCT147650.07.844367
TCGGAAG679850.07.742386370
TCGAATC106450.07.560652338
CGGAAGA659600.06.827166670
ACGCCGT119150.06.637378732
GTCGAAT124350.06.50052837
CGAATCT125650.06.32170339
CAGATCG363200.06.252405670
Produced by FastQC (version 0.11.5)