FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55959967
Sequences flagged as poor quality0
Sequence length34-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2481920.4435170592577369No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1001770.1790154736867518No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG947000.16922811980929153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG94850.030.6250066
TCGACGA102500.028.5368987
CGACGAG105200.027.3553358
CGGTATA156700.026.39044420
GTTCGAC124100.023.4066985
ACGAGGA164600.023.06070110
GAACGAT564650.022.40612245
GACGAGG175250.021.7106519
CAGTTCG139350.021.5548823
GTATAGA210500.020.82126222
ATAGAGT209950.020.76899324
CGAGGAT166900.020.70723711
TATAGAG220800.020.04374923
AGTTCGA149450.019.6766454
GACCGCG639950.019.443075
ACCGCGT641900.019.408616
AGACCGC653300.019.224214
ATGACGG199200.019.1334616
CCGCGTT655050.019.0258547
CAGACCG664050.018.9875413
Produced by FastQC (version 0.11.5)