FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80476822
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA869150.010.88874494-95
ACACGTC1270950.010.21125494-95
ACGTCTG1041600.09.79795794-95
CACGTCT1218400.09.63098194-95
CACACGT1498250.09.49023794-95
GCACACG1734050.09.01394894-95
ATCGGAA3958750.08.49477594-95
TCGGAAG3720900.08.4720694-95
CGGAAGA3485750.08.45115794-95
GATCGGA4345200.08.34909494-95
AGCACAC2146500.08.23202394-95
AGATCGG4828100.08.23122794-95
GTCTGAA1096500.08.20444594-95
AGAGCAC2587500.08.17826694-95
TCTGAAC989150.08.17498894-95
GAGCACA2453450.08.02289394-95
TAGATCG1347850.08.00342994-95
CAGATCG1678600.07.493548494-95
GAAGAGC3433700.07.40717594-95
AAGAGCA3115050.07.40672194-95
Produced by FastQC (version 0.11.5)