FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56045183
Sequences flagged as poor quality0
Sequence length26-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG204350.012.40107745
GGGAGGT339100.011.52041153
GGAGGTC328750.011.4580974
ATAGGGG227250.011.44560638
GGCATAG221450.011.36968835
GTCACTC311050.011.3566758
GGTCACT318000.011.158117
AGGTCAC337450.011.0217996
GAGCTTA243800.010.50474744
ACTCTCC348600.010.2356211
GAGGTCA372050.010.196725
TGGCATA252050.010.16821634
CATAGGG268900.09.59741737
GTTGTCG35100.09.35806526
TCACTCT395550.09.1865189
GCATAGG281200.09.08959136
ATATAGC42900.09.0211156
CACTCTC403200.08.84452710
TCAGACG90300.08.6476452
TGCCCGT196900.08.60649644
Produced by FastQC (version 0.11.5)