FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80589807
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA703450.010.90116394-95
ACGTCTG852250.09.9804294-95
ACACGTC1029250.09.68230294-95
CACGTCT993050.09.29596694-95
CACACGT1215900.09.05215294-95
ATCGGAA3231100.08.71932194-95
GCACACG1432150.08.70654394-95
GATCGGA3546300.08.63412894-95
TCGGAAG3054450.08.57106794-95
AGATCGG3953850.08.48759594-95
CGGAAGA2877300.08.47285194-95
TAGATCG1140450.08.10203394-95
AGAGCAC2172300.08.02126194-95
AGCACAC1817950.07.876337594-95
CAGATCG1393900.07.68223294-95
GAGCACA2083400.07.67391494-95
AAGATCG1241200.07.57838794-95
GTCTGAA960800.07.36045794-95
GAAGAGC2940650.07.280971594-95
AAGAGCA2654350.07.205219394-95
Produced by FastQC (version 0.11.5)