FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60444219
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA725650.12005283747648389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG169350.012.71476838
GGCATAG166100.012.54411135
AGCTTAG181600.011.63625945
GGGAGGT272000.011.0922413
AACTCTA159100.010.9479945
CATAGGG199550.010.61019137
GTTGTCG33200.010.5039626
GGAGGTC277500.010.4994424
GTTCTAT199900.010.3321171
GAGCTTA205200.010.28700844
GTCACTC270400.010.2512058
GCATAGG210350.010.0122336
GGTCACT281400.09.8264967
TGGCATA219100.09.71510134
AGGTCAC300950.09.6888826
TCTATAG325200.09.5958923
ACTCTCC300850.09.52027111
GAGGTCA315100.09.3108365
CACCGAA326700.09.27170345
GCACCGA327900.09.18286844
Produced by FastQC (version 0.11.5)