FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56900535
Sequences flagged as poor quality0
Sequence length49-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG52450.029.026216
TCGACGA63200.024.2685017
CGGTATA92050.023.867320
CGACGAG63300.023.8358848
GTTCGAC65750.023.2237665
GACCGCG142450.017.9982095
ACCGCGT142700.017.918986
AGTTCGA87500.017.8140224
CAGTTCG91900.017.8005353
GTATAGA134450.017.59364522
AGACCGC149500.017.301234
ACGAGGA118450.017.23916810
ATGACGG119100.017.14511316
TCGGCGC103700.016.81073445
AACGACG39900.016.55246516
ATAGAGT146250.016.43521524
CCGCGTT155650.016.38447
CGCGTTC158450.016.1521438
GACGAGG122650.016.0385279
GACGGTA142350.015.59149318
Produced by FastQC (version 0.11.5)