FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56900535
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA138050.013.70701645
AGCTTAG167900.012.95662645
ATAGGGG176150.012.56128838
GGCATAG177000.012.19708135
GGGAGGT275600.011.1740683
TATATAC75000.010.9232377
CATAGGG201500.010.89244337
GGAGGTC270300.010.8305144
GTCACTC266750.010.4727098
GAGCTTA208050.010.46702644
GCATAGG210300.010.38323736
CATGCAT109750.010.1733969
ACTCTCC280550.010.14423811
GGTCACT278350.010.044427
ATATATA87950.010.0115496
GAGGTCA308200.09.8079215
TGGCATA225350.09.77951334
AGGTCAC301550.09.7532886
TCAGACG101250.09.406412
CCATATA91750.09.0527474
Produced by FastQC (version 0.11.5)