FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57103561
Sequences flagged as poor quality0
Sequence length48-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1851540.3242424758764169No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT904900.15846647462143384No Hit
CTCCTGTGTAACTGGTTTTGTTGGATACTCGTCAGCTGTGAGCCGATCACT815310.14277743554381836No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA665940.11661969732500571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG49600.025.7664226
CCGGCTT460150.023.1295642
GCCGGCT447250.022.1817341
TCACGCT454500.022.1608438
TTCACGC457900.022.0503397
CGGTATA77550.021.52550120
TCGACGA59700.021.4045987
GTTCGAC59650.020.9725785
CGGCTTC526500.020.5344223
ACGCTCA506350.020.43356510
AACGCTA501400.020.16863623
ACGCTAC506150.019.96602824
CGCTACC514900.019.61799625
ACCGCGT231450.019.3067676
AGACCGC232250.019.2790154
CGACGAG64900.019.0656268
GACCGCG233800.019.0453415
AAAACGC533400.019.0303721
GAAAACG538500.018.72480620
AAACGCT543000.018.67320622
Produced by FastQC (version 0.11.5)