FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57103561
Sequences flagged as poor quality0
Sequence length50-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1527680.2675279743061908No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA927870.1624889908354402No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG751570.13161525951069847No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG596890.10452763182317124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT663050.016.2069073
GGAGGTC671750.015.7792924
AGCTTAG484050.015.60957945
GTCACTC689750.014.9630168
GGTCACT700950.014.7849197
AGGTCAC726950.014.4758796
GAGGTCA738700.014.3887765
TGGGAGG559700.014.15846352
ACTCTCC731750.014.14723511
ATAGGGG538100.014.1414138
GGCATAG540600.013.98028635
GAGCTTA556050.013.644443544
TCACTCT779500.013.3296859
CTGGGAG635950.013.2887681
TGGCATA576150.013.10204334
CACTCTC787050.013.09318410
AACTCTA122100.013.01037345
TATATAC55750.012.5111987
TCTCCCC859250.012.08727313
AGGCTCT646850.011.98653920
Produced by FastQC (version 0.11.5)