FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78558669
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1816950.09.51356694-95
ACACGTC572800.09.49902194-95
GATCGGA2010050.09.44410294-95
CGTCTGA399250.09.34047994-95
AGATCGG2285300.09.22467394-95
TAGATCG763500.09.08168494-95
TCGGAAG1779300.08.99411394-95
CACACGT715000.08.84725194-95
CGGAAGA1692700.08.50423694-95
ACGTCTG496700.08.33762694-95
CACGTCT586100.08.30496494-95
CAGATCG917300.07.95485394-95
GCACACG872450.07.92445894-95
AAGATCG636200.07.18891194-95
AGAGCAC1402900.07.10359494-95
GAGATCG736150.06.604327794-95
AGCACAC1179350.06.55139894-95
GAAGAGC1984150.06.38503194-95
GAGCACA1376600.06.37678894-95
AAGAGCA1756750.06.342079694-95
Produced by FastQC (version 0.11.5)