FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79494192
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1888500.09.55787994-95
GATCGGA2095450.09.35084494-95
CGTCTGA433450.09.19983594-95
AGATCGG2374850.08.92407694-95
TCGGAAG1856750.08.89433594-95
ACACGTC617550.08.74700794-95
TAGATCG701750.08.69470494-95
CGGAAGA1771350.08.28651694-95
ACGTCTG538000.08.06238894-95
CACACGT769050.08.02030794-95
CAGATCG948250.07.703094594-95
GCACACG945250.07.690832694-95
CACGTCT631800.07.48787294-95
AAGATCG699350.07.289747794-95
AGAGCAC1468300.07.058427394-95
AGCACAC1246300.06.608031794-95
GAGCACA1452650.06.60296894-95
AAGAGCA1810400.06.49452894-95
GAGATCG817000.06.41697794-95
GAAGAGC2066000.06.19072394-95
Produced by FastQC (version 0.11.5)