FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63770940
Sequences flagged as poor quality0
Sequence length50-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG46650.023.9225946
TCGACGA55000.020.1679847
CGGTATA83800.020.0564720
CGACGAG54550.020.0456168
ACCGCGT104600.017.9180836
AGACCGC111900.016.7494534
ACGTTCG22300.016.1433376
GACCGCG114750.016.1177675
CCGCGTT116500.015.991267
CAGACCG119400.015.9046413
CGCGTTC117600.015.8990678
AACGACG36650.015.34772216
CGTTACG27450.014.99984121
CCCGTAT36150.014.7514481
ATGACGG111500.014.36755916
AACGCTA110600.014.34207223
GTATAGA125700.014.33755722
ACGCTAC113400.014.02762924
GTTCCTA91750.013.97806415
ATAGAGT130000.013.74216224
Produced by FastQC (version 0.11.5)