FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005744437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79538808
Sequences flagged as poor quality0
Sequence length25-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA573250.09.87477194-95
ACACGTC832700.09.26686894-95
ACGTCTG703950.09.17660694-95
CGTATCA68600.09.1020092
ACGTATC64900.09.0378351
GATCGGA2837100.08.92910994-95
ATCGGAA2568450.08.87438694-95
CACGTCT821850.08.74799494-95
AGATCGG3186200.08.61599594-95
CACACGT1009000.08.61477394-95
TCGGAAG2478050.08.48069194-95
TAGATCG915450.08.47349394-95
CGGAAGA2349850.08.31001494-95
GCACACG1232100.08.08654694-95
CAGATCG1196150.07.833344594-95
AGAGCAC1849800.07.74200994-95
AGCACAC1564800.07.22951194-95
AAGATCG940300.07.177945694-95
GAGCACA1776500.07.12263394-95
AAGAGCA2234850.06.89396794-95
Produced by FastQC (version 0.11.5)