FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59795100
Sequences flagged as poor quality0
Sequence length50-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTCT619100.1035369118874289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG39750.021.3958576
GTTCCTA145650.020.29856715
CCTACTC167950.017.73707218
CGGTATA69950.017.56219720
TCGACGA51650.017.2068677
AGTTACC176700.016.70596534
CGACGAG52350.016.6329528
CCCGTAT42750.015.63238051
GTCCGTT42650.015.6162751
AACGCTA90650.014.84250923
CGAGCTA36750.014.816256511
GTTTACG41750.014.7662126
GTTCGAC57500.014.7527215
AACATGC230750.014.6755175
CTACTCT205650.014.55113519
AACGACG41550.014.51236116
GCCCTGT209600.014.48112310
TTACGAG41650.014.4775418
ACGCTAC94150.014.33853924
TACTCTC212050.014.30294820
Produced by FastQC (version 0.11.5)