FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005744442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005744442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74180186
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGGCCA2058595227.751281184439197No Hit
GAGACTAT1691262622.79938473058021No Hit
CGCTTAGC1573361021.20998995607803No Hit
TCTCAGTG1548573520.87583738331419No Hit
TCCCAGTG5097370.6871605849033595No Hit
ACAGGCCA3192100.43031706606936787No Hit
GAGACCAT2773020.3738221955927692No Hit
CGCCTAGC2591980.349416756652511No Hit
AGAGGCCA2417670.325918568066141No Hit
GAGACTAA2156510.2907124012873195No Hit
GAGACTAC2000880.2697324053622621No Hit
TCTCAGCG1985800.2676995174964916No Hit
AAAGGCCA1863960.25127464630514673No Hit
CGCTCAGC1831370.24688128983661486No Hit
NTAGGCCA1816550.24488345176163348No Hit
CGCGTAGC1693930.22835343119792123No Hit
CCTCAGTG1687870.2275365014587588No Hit
TCTCAGGG1614430.21763628362970133No Hit
GAGACTAG1482890.199903785628146No Hit
NAGACTAT1439220.19401676884444588No Hit
NGCTTAGC1363540.18381458358705113No Hit
NCTCAGTG1333230.1797285868223625No Hit
ATAGGCAA1095680.14770521066097084No Hit
TCTCAGAG989320.13336715009045677No Hit
CGCTGAGC975420.1314933343521139No Hit
TCTCGGTG908580.12248284198155017Illumina Single End PCR Primer 1 (100% over 8bp)
TCTCAGTA905220.12202989083904425No Hit
GAGACGAT819450.11046750408525531No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)