FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397498
Sequences flagged as poor quality0
Sequence length2-144
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTAACAAACCCTGCTTCTTTATATTCAGGAGATGTTCTGGACTCACA46191.1620184252499384No Hit
ACAATAACAAACCCTGCTTCTTTATATTCAGGAGATGTTCTGGACTCACA13120.3300645537839184No Hit
ACAGTAACAAACCCTGCTTCTTTATATCATTCATTCAACTGACCTTTGTC9990.2513220192302854No Hit
TGACAAAGGTTAGTTGAATGAATGATCACTGTAGAGCACCTCCCTATTTT8780.2208816144986893No Hit
TGATAAAGGTTAGTTGAATGAATGATCACTGTAGAGCACCTCCCTATTTT8220.2067934933005952No Hit
ACAGTAACAAACCCTGCTTCTTTATATCATTCATTCAACTAACCTTTGTC7130.17937197168287639No Hit
TGACAAAGGTCAGTTGAATGAATGATCACTGTAGAGCACCTCCCTATTTT6910.17383735264076802No Hit
TGATAAAGGTCAGTTGAATGAATGATCACTGTAGAGCACCTCCCTATTTT5860.14742212539434163No Hit
ACAGTAACAAACCCTACTTCTTTATATTCAGGAGATGTTCTGGACTCACA5300.13333400419624752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTTT4200.0249.37224138
GACGCAA601.4551915E-1189.732218
CAATAAC3050.088.261192
TAAAGGT2650.083.80654
GAGACGC653.092282E-1182.8297356
ACGCAAG705.820766E-1176.913329
AGACGCA751.07320375E-1071.785777
ATAACAA3850.069.9211964
ACAATAA3950.069.854821
CGACCAG150.002598976567.3076336-37
CTTACGT150.002600273167.2991614-15
AATAACA4100.065.657713
AGGAGCT13750.063.145832136-137
ATAAAGG3550.062.5597843
GGAGCTT5800.057.803734136-137
ACAAACC23600.052.7556957
CAAACCC23750.052.1391378
AAACCCT24600.050.6111569
GAGTTAC9300.050.48417100-101
TTGCGTC200.00811719150.48071736-37
Produced by FastQC (version 0.11.5)