FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853507
Sequences flagged as poor quality0
Sequence length2-144
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAA10510.12313900178908901No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG400.005943525153.78394138
GGCAGGT1053.484456E-8146.46089138
TCGGGAG100.00797463139.41179136-137
CGTTACT100.008965702134.054183
GGCAGGC904.881613E-4102.52263138
GTTTACA13550.095.470336
TCACAGT16450.079.047161
AGTTTAC18650.069.363165
GGAGAAT9000.067.7718357
CACAGTT19400.067.02712
TTTACAT22850.062.48047
GACAGGG1000.00899807861.513573138
AAGACAG10400.059.14767138
CACAATT2400.058.6487052
GAGAATG11200.056.2548838
TTACATT25950.055.5330478
AACAGTT4300.053.776005126-127
TGCAACA3850.053.358994112-113
AACTCAC6800.052.75618458-59
CGAACAA2550.051.2710354-55
Produced by FastQC (version 0.11.5)