FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853507
Sequences flagged as poor quality0
Sequence length2-144
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAA11350.1329807488397869No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT100.008952318134.121227
GTGGCGG100.008952318134.121222
CGGCATT259.525002E-6107.296961
GGCAGGC1302.4876135E-7105.35503138
GACAGGG1202.1190186E-591.30769138
TCACAGT15800.078.5203251
GGCAGGT1901.1799784E-872.085014138
GTTTACA17900.070.4323656
TGCGGAG300.002637203567.06061
GGTAGGT1306.9031375E-763.213013138
CGGAATC451.6474587E-857.974365134-135
AGCAATT4300.056.324898126-127
AGTTTAC22350.056.108875
CACAGTT22750.055.122342
GGAGAAT18250.053.6484877
TGAACCG252.9557542E-453.6484812-13
CGTATTC700.053.49429796-97
ACGAATT1850.052.56101230-31
GCCGGAA504.0448867E-851.733948132-133
GTTTCCG655.456968E-1251.6546866-67
Produced by FastQC (version 0.11.5)