FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880247
Sequences flagged as poor quality0
Sequence length30-144
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTTTTTTT189141.0059316674883672No Hit
ACCCATCCACACCTTCCTGTTTTCCACAGAACAGCCAAACAAACTTTTCA144920.7707498004251568No Hit
ACCCATCCACACCTTCCTGGTACATCTTGAACCCACAAGGCAGGCACTAG108630.5777432433079271No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCGTGATTTCCACTTGGCCAGACCGTC55980.29772684120756476No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGCAGTTTTTCTTTCC42480.22592776374593337No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTTTTTTC41720.22188574160735267No Hit
ACCCATCCACACCTTCCTAGTACATCTTAAACCCACAACTACATGTGAAG32800.17444516598085252No Hit
GGGTAGAAAGCAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTTTTTTT28860.15349047226242085No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTTTTTTT28780.1530649962478334No Hit
ACCCATCCACACCTTCCTAGTACATCTTAAACCCACAAAACAAACACTAA25890.13769467522086193No Hit
ACCCATCCACACCTTCCTGATACATCTTAAACCCACAAGACAGACACTAA24690.13131253500205026No Hit
ACCCATCCACACCTTCCTAATACATCTTGAACCCACAAGGCAAACACTAG23740.12626000732882436No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGCAGTTTTTTTTTCT21780.11583584497143193No Hit
ACCCATCCACACCTTCCTAATACATCTTAAACCCACAAGGCAGACACTAA19550.10397570106480691No Hit
ACCCATCCACACCTTCCTAATACATCTTAAACCCACAAGACAAACACTAG18870.10035915494081363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACC8750.0230.21904138
TGGAACC4700.0166.67749138
TGGTTCG100.008717843135.321781
ATCCACA94250.0128.860785
CATCCAC94100.0128.706684
TCCACAC96300.0126.468946
CCACACC96850.0125.331587
GAACCGC600.0124.044964
AGCACAT7750.0116.86746136-137
GTAGAAA91150.0116.170363
GGGTAGA93050.0113.143841
AGTAAAG82550.0112.9457249
CACATTT3508.913048E-11111.91203138
GCGAAAC352.0677726E-7108.73023136-137
ACCCATC113450.0106.635241
ACACCTT127300.095.937099
CACACCT127950.095.396838
AGCAAAG14550.089.284479
GGTAGAA119700.088.405712
CCGCTCT700.086.992577
Produced by FastQC (version 0.11.5)