FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3287526
Sequences flagged as poor quality0
Sequence length4-144
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCATCCACACCTTCCTATCTTCCACAAAGCAACCAGACAAACTTTTCA96160.2924995878359593No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTTTTTTT63190.19221140760559763No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTTTTTTC46330.1409266421010815No Hit
ACCCATCCACACCTTCCTGGTACATCTTGAACCCACAAGGCAGGCACTAG44290.13472136798309733No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTCTTTTC40490.12316252403783272No Hit
ACCCATCCACACCTTCCTGATACATCTTAAACCCACAAAACAAACACTAA38790.1179914622728459No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTTTTTTT38600.11741352007558267No Hit
ACCCATCCACACCTTCCTGATACATCTTAAACCCACAAGACAAGCACTAG34850.10600676618222943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACC10400.0253.98245138
CACATTT7250.0182.16672138
TGGAACC16250.0154.25545138
ATCCACA132450.0129.867545
CATCCAC132400.0129.865974
CCACACC136900.0125.646147
TCCACAC137200.0125.37146
ATGGGAC6950.0104.447876136-137
AGCACAT14150.0103.51049136-137
GTAGAAA89600.0101.8581543
AGTAAAG80950.099.663549
GGGTAGA95400.095.033371
ACCCATC189300.091.397081
TGAGACC16200.089.844124138
TAGGACC11950.087.96455138
AATGGGA13150.087.933174136-137
ACACCTT199450.086.779499
CACACCT199650.086.625438
TAGAAAG123350.073.988584
CGGTGAA1950.072.96868134-135
Produced by FastQC (version 0.11.5)