FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3287526
Sequences flagged as poor quality0
Sequence length4-144
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTTTTTTT81330.24738967843904502No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTTTTTTC60150.18296433244938595No Hit
ACCCATCCACACCTTCCTATCTTCCACAAAGCAACCAGACAAACTTTTCA60100.18281224239747457No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTCTTTTC53280.16206715931676283No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTTTTTTT46650.1419000184333143No Hit
ACCCATCCACACCTTCCTGGTACATCTTGAACCCACAAGGCAGGCACTAG43290.1316795669448698No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGTAGTTTTTTTTTTC36140.10993068952154296No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGCAGTTTTTCTTTCC35950.10935274732427971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACC9150.0225.94865138
CACATTT8000.0181.23578138
GATGGGG601.6716102E-4134.24873138
CCATCCG100.008974504134.015963
CATCCAC112100.0128.39714
ATCCACA113300.0127.74695
TGGAACC14400.0126.79046138
TCCACAC117100.0123.773056
CCACACC117350.0123.223877
CGGCATT650.0113.3981251
GTAGAAA104050.0107.290053
AGTAAAG94650.0104.848199
TGAGACC13700.0103.87128138
ATGGGAC5650.0102.42933136-137
AGCACAT15950.0101.997635136-137
GGGTAGA116000.095.717421
ACCCATC159250.090.2556461
ACACCTT173150.084.635559
TAGGACC12200.083.63035138
CACACCT175400.083.320648
Produced by FastQC (version 0.11.5)