FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7767064
Sequences flagged as poor quality0
Sequence length2-144
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCACC121700.0123.823789
GCCTCTT122800.0112.079713
CAGCCTC129400.0106.3108441
AGCCTCT133250.0103.188412
CCTCTTC165200.091.546534
TTCTCAC192300.078.997168
GAACTTA116400.078.595581
ACTTAGG118000.077.30053
CTTAGGA119550.076.298274
GCACACG209.3812414E-567.66704624-25
CGGAGTA300.002545584267.666614
AACTTAG136000.067.56712
GAAGTAA189750.067.256519
TTCAGGG84750.067.04976138
ACGATTG58500.065.0684446-47
ACCTCTT18200.065.064053
TAACGAT58700.064.9033444-45
TCACCAC118850.063.99433510-11
CACACGC67050.063.8825424-25
TTAACGA58900.063.706444-45
Produced by FastQC (version 0.11.5)