FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555831
Sequences flagged as poor quality0
Sequence length1-144
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTAACAAACCCTGCTTCTTTATATTCAGGAGATGTTCTGGACTCATA95881.7249847525596809No Hit
ACAATAACAAACCCTGCTTCTTTATATTCAGGAGATGTTCTGGACTCATA31700.5703172367140372No Hit
TGACAAAGGTTAGTTGAATGAATGATCACTGTAGAGCACCTCCCTATTTT21420.3853689340824819No Hit
TGATAAAGGTTAGTTGAATGAATGATCACTGTAGAGCACCTCCCTATTTT20210.3635997272552269No Hit
TGACAAAGGTCAGTTGAATGAATGATCACTGTAGAGCACCTCCCTATTTT15880.28569835075769434No Hit
TGATAAAGGTCAGTTGAATGAATGATCACTGTAGAGCACCTCCCTATTTT12580.22632778668336237No Hit
ACAGTAACAAACCCTACTTCTTTATATTCAGGAGATGTTCTGGACTCATA10100.1817099082275008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTTT5650.0322.444138
ACAGTAA12750.0123.533991
CAGTAAC12750.0123.0060652
GTAACAA12750.0122.478144
AGTAACA13150.0119.264433
TAACAAA17500.0116.92745
ACAAACC17400.0116.825737
CAAACCC17750.0113.7636958
AAACCCT18200.0110.950859
AACAAAC19300.0105.3247456
CAATAAC4550.0103.554132
AAGGTTA5700.099.193956
AGGTTAG5800.098.644237
GTTAGTT5750.097.160789
CAAAGGT5450.092.628694
ACAATAA5300.088.9002461
GATAAAG4500.088.251132
AAAGGTC3900.086.295115
AAAGGTT6500.085.949925
TGATAAA4550.085.8019941
Produced by FastQC (version 0.11.5)