FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3192239
Sequences flagged as poor quality0
Sequence length2-144
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAAAGGTGGACAGAAGGGTCGGGTTTGGTGTGGAGGGTGAGCAAGAGG86360.27053112251307No Hit
AAGAAAGGTGGACAGAAGGGTAGATACGTTTTATTTTTGAATTATAGTTT76430.23942442906060607No Hit
AAGAAAGGTGGACAGAAGGGCAGATACGTTTTATTTCTGAATTACAGTTT56170.1759580031445014No Hit
CCCAAATGCTACACCTCCAATAAAAAAAAACTCAAGAAATAAAAGAAAAA54580.17097717307507362No Hit
AAGAAAGGTGGACAGAAGGGGCCAGCCTGCAAGGTGGATTCCAATTACCC40000.12530390111767947No Hit
AAGAAAGGTGGACAGAAGGGTGGGGGATGTTCCACTTTCTTTGTGAATGT39820.12474003356264991No Hit
AAGAAAGGTGGACAGAAGGGTAGATACGTTTTATTTTTGAATTACAGTTT37750.11825555667981001No Hit
CCCAAATGCTACACCTCCAGTAAAAAGGAACTCAAAAAATAAAAGGAAAA37090.11618804231136828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCC47550.0244.5926138
AGTTCAG49900.0156.28848138
GTGGACA111600.0127.104878
ATGCTAC80250.0124.006936
TGCTACA85550.0116.5611957
CAAATGC85200.0116.485333
TGGACAG124200.0114.3732459
GGCGGAC800.0109.710177
GGTGGAC129550.0108.814457
CTACACC92800.0106.656259
GCGGACA950.0106.602648
CGGACAG1000.0101.2725149
GCTACAC106550.093.5261848
CCCAAAT113450.087.360481
GAAAGGT162250.087.216783
AATGCTA116350.085.531215
AAATGCT122900.081.357334
AAGGTGG175600.080.778365
CCAAATG124850.079.275492
ATCTAAT79750.076.94732136-137
Produced by FastQC (version 0.11.5)