FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651227
Sequences flagged as poor quality0
Sequence length16-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTAACAAACCCTGCTTCTTTATATTCAAAAGATATTCTGGACTCACA10430.16015920715817986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTT2700.0166.37384138
CGCTTAT801.3962848E-4140.37793138
GCATGGT151.6611666E-4134.4577
AAGCTTT4750.0118.213138
CAGTAAC12350.0112.138232
CAATAAC4300.0109.441742
AGTAACA13550.0102.703323
ACAGTAA13750.0100.231581
AAGGTTA9100.095.301936
GTCAGTT7000.095.080319
TAAAGGT7200.094.306654
CAAAGGT9300.092.529564
ACAAACC20950.089.210147
AGGTCGT1950.086.63274136-137
AAAGGTT10450.082.9902045
AAGGTCA8300.080.998196
AACAAAC23400.079.869766
TGATAAA8800.076.396031
GTAACAA18250.076.25374
TAACAAA24700.075.3939065
Produced by FastQC (version 0.11.5)