FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265826
Sequences flagged as poor quality0
Sequence length31-144
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAA53070.23421922071686No Hit
TGTGGAGGAGAATGAATGGTTTCTATGGTGACGAATTAGTCATTCTTATT35760.15782323973685533No Hit
TCACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGATTTAAAAA29370.12962160377716558No Hit
TCACAGTTTACATTCCCCAAAGAAAATTAACTAATTAAAAGGTTTAAAAA27020.11925011011436888No Hit
TCACAGTTTACATTCCCCAAAGAGAATTAACTAATTGAAAAGTTTAAAAA26410.11655793516360038No Hit
TCACAGTTTACATTCCCCAAAAAGAATTGACTAATTGAAAAATTTAAGAA25700.11342442005696819No Hit
TCACAGTTTACATTCCCCAAAAAAAATTAACTAATCAAAAAATTTAAGAA24530.10826074023336302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGGC1650.0247.264138
GGCAGGC1106.366463E-11202.30692138
GGCAGGT1051.14123395E-8176.61716138
GCGATAA100.0086733345135.554328
CACCGTT100.0086733345135.554322
GATTAGT23300.0117.7953138
GTTTACA66150.098.771266
TCACAGT67150.097.502221
GGAGAAT73200.097.2213447
GGTAGGT5800.095.92139138
CACAATT15550.093.275322
GAGAATG77850.091.240178
CACAGTT73450.089.1392062
AGTTTAC77950.083.819365
AGGAGAA90300.078.060086
TGTGGAG91300.076.611211
TTTACAT106650.075.244417
TTACATT108100.074.5486158
GATCAGT23150.073.69856138
GAGGAGA96850.072.850825
Produced by FastQC (version 0.11.5)