FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764859
Sequences flagged as poor quality0
Sequence length2-144
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCATCCACACCTTCCTGGTACATCTTGAACCCACAAGGCAGGCACTAG62410.35362598371881265No Hit
ACCCATCCACACCTTCCTATTTTCCACAAAGCAGCCAAACAAGCTTTTCA30340.17191175045711868No Hit
ACCCATCCACACCTTCCTTATTCCTACACAGAATGTTACTAAACACTTAA28080.15910619488582373No Hit
ACCCATCCACACCTTCCTGTCTTCCACAAAGCAGCCAAACAAGCTTTTCA26040.14754719782146902No Hit
ACCCATCCACACCTTCCTTATTCCTACACAGAATGTTACTAAACACTTGA24240.13734808276468546No Hit
ACCCATCCACACCTTCCTGGTACATCTTAAACCCACAAAACAAACACTAG22620.12816887921358022No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTTTTTTT18410.10431428233076975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACT255.5159326E-6315.77087138
TGGGACC5250.0215.52617138
TGGAACC6250.0189.46252138
ACTAACT350.00635956150.36708138
TGAGACC8700.0127.03426138
CATCCAC82350.0125.9681854
ATCCACA82800.0125.365145
TCCACAC85000.0122.199876
CCACACC85200.0121.913017
CACATTT5000.0115.78265138
GCGAAAC153.6124364E-4110.6252136-137
GGGTAGA38050.0104.542751
TAGGACC6600.099.675156138
ACCCATC114900.090.4002461
CACACCT116950.089.0466168
ACACCTT121100.086.273919
GTAGAAA49450.085.0853963
TAAGACC11500.082.375015138
AGTAAAG47950.073.9441769
ACAGCGA308.2609404E-872.2136134-135
Produced by FastQC (version 0.11.5)