FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764859
Sequences flagged as poor quality0
Sequence length2-144
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCATCCACACCTTCCTGGTACATCTTGAACCCACAAGGCAGGCACTAG52940.29996730617006795No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGTAGTTTTTTTTTTT28750.16290253215695985No Hit
GGGTAGAAAGTAAAGAGAGAGTGGTATGTTTTGAGGCAGTTTTTCTTTTC21410.12131280742540905No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGCAGTTTTTTTTTTC21020.11910299916310595No Hit
ACCCATCCACACCTTCCTTATTCCTACACAGAATGTTACTAAACACTTAA19850.11247357437619662No Hit
GGGTAGAAAGTAAAGAGAGAGTGGCATGTTTTGAGGTAGTTTTTTTTTTC19060.10799729610127494No Hit
ACCCATCCACACCTTCCTTATTCCTACACAGAATGTTACTAAACACTTGA18000.10199115056783574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACC4900.0222.07864138
TGGGACT350.0053373086159.44107138
TGGAACC3700.0143.28151138
CATCCAC57550.0123.286564
ATCCACA59150.0121.777775
TCCACAC61000.0118.3058556
CCACACC60900.0117.8357
GGGTAGA53500.0103.218581
GTAGAAA58600.097.461053
ACCCATC78550.089.9826661
CACATTT5350.088.66116138
AGTAAAG55150.088.1343849
AATCCAC1150.088.054544
TGAGACC7050.087.07066138
GCGAAAC209.956537E-485.72214136-137
ACACCTT87600.083.53799
CACACCT87200.082.992088
CCCATCA1100.079.782752
TAGGACC6300.079.72054138
AGCAAAG11650.075.910839
Produced by FastQC (version 0.11.5)