FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712660
Sequences flagged as poor quality0
Sequence length2-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA554.4325498E-7198.64311138
CGCCTAT554.4325498E-7198.64311138
CGCTTAT350.005685948156.07674138
GGCATTC151.6767715E-4134.142992
GAGATCG151.6767715E-4134.142999
TGATAAA8700.0104.84741
CAATAAC5550.0103.93062
ACAGTAA11550.0100.462071
CAGTAAC11750.098.7520752
AGTAACA14400.081.0447163
GGTTCAC250.001278484780.485791
AAGCTTT4350.075.34739138
ACAAACC24250.073.018047
TGACAAA13050.071.9540941
CAAAGGT12900.071.75094
TAAAGGT13250.068.3374
AAGGTTA15200.067.0714956
GAGCGTC150.002635869467.07149518-19
AGAGCGT150.002635869467.07149518-19
CCGCTTA300.00267661866.809006136-137
Produced by FastQC (version 0.11.5)