FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387089
Sequences flagged as poor quality0
Sequence length22-144
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAA200831.4478523007535926No Hit
TGTGGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGAC103060.7429948619014354No Hit
GGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGACAGT17270.1245053489718396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGGC7150.0157.04344138
ACAGTTT21800.0123.750043
GTTTACA22050.0121.732176
TTTACAT22950.0117.8444067
GGAAGAC20450.0113.1039136-137
GAGAATG16500.099.825428
GGAGAAT17300.095.2092067
AGTTTAC39050.069.605265
CGGTTTA150.002527812567.78512640-41
GTTCGGA300.002527906867.7826849
AGTTCGG300.002527906867.7826848
TTACATT41550.065.580368
AAGACAG24450.064.76569138
CACAGTT45400.059.869732
ATTCCCC22750.059.29122512-13
AGAGAAT24300.055.64874620-21
AAACGAG24400.055.42267650-51
CAAAGAG24250.055.34421518-19
ACGAGCA24450.055.30933452-53
GTTCCCG13000.055.28191466-67
Produced by FastQC (version 0.11.5)