FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387089
Sequences flagged as poor quality0
Sequence length22-144
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAA271391.9565435238834712No Hit
TGTGGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGAC180531.3015026433055126No Hit
CACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAAA32550.23466410590812845No Hit
GTGGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGACA21710.15651483069939995No Hit
GGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGACAGT16620.11981927619640845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGGC9550.0173.51541138
ACAGTTT33100.0113.2034153
CACCGTT301.6494232E-7112.930472
GTTTACA34050.0110.2440266
TTTACAT35000.0108.606847
GCGAATG205.059837E-4101.637438
GAGAATG30900.0101.089228
GGAGAAT31100.0100.4391257
ACCGTTT354.1330532E-796.797553
GGAAGAC30100.078.12949136-137
AGTTTAC49350.076.339625
TTACATT54450.071.42938
AAACGCG209.31586E-567.7607350-51
AACGCGC209.31586E-567.7607352-53
ACGCGCA150.002531424467.7607252-53
CCGTTTA503.447094E-667.758284
CACAGTT58300.064.736472
AGAATGA48900.063.601339
TACATTC63500.061.3559279
AAGACGC401.1308657E-860.807537122-123
Produced by FastQC (version 0.11.5)