FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005745478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005745478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016649
Sequences flagged as poor quality0
Sequence length7-144
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTTTACATTCCCCAAAGAGAATTGACTAATTGAAAGGTTTAAGAA150331.4786814328248983No Hit
TGTGGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGAC84270.8288996497316183No Hit
GGAGGAGAATGAATGGCATCTATGGCCTTAATTTTGCCAGCAAAGACAGT13130.12914978522577605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGT50.00242555891836.8143138
AGACGCG100.008732917135.240556
GGCAGGC5550.0132.38301138
GTTTACA16900.0119.235766
ACAGTTT17300.0116.478873
TTTACAT18050.0115.010677
GGAGAAT15200.087.194577
GAGAATG15550.084.7971348
GGAAGAC12900.080.011734136-137
CACAGTT27050.074.994762
AGTTTAC28750.070.325095
AAGACAG18450.069.68943138
TTACATT31050.066.8580558
ATTCCCC17750.058.28677712-13
TCACAGT35150.057.3281441
TACATTC36700.056.9336979
CAAAGAG19100.054.34404818-19
ACGAGCA19150.054.20748552-53
AAACGAG19100.053.99267250-51
AAAAACG19300.053.4331648-49
Produced by FastQC (version 0.11.5)